Green Flourescent Protein Methods in Enzymology, vol. 302: P. Michael Conn (Editor)

Description

This Methods in Enzymology volume deals with the utility of green fluorescent protein (GFP). The OVID database (including MEDLINE, Current Contents, and other sources) lists nine references to GFP for the ten-year period 1985-1994. In contrast, in less than four years thereafter, more than 500 are listed, a testament to the rapid growth of interest in this probe.
This volume documents many diverse uses for this interesting molecule in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in the sources from which they were taken. The techniques are described in a context that allows comparisons to other related methodologies. The authors were encouraged to do this in the belief that such comparisons are valuable to readers who must adapt extant procedures to new systems. Also, so far as possible, methodologies are presented in a manner that stresses their general applicability and potential limitations. Although for various reasons some topics are not covered, the volume provides a substantial and current overview of the extant methodology in the field and a view of its rapid development.
Table of Contents
Membrane trafficking. (3-11)
Kupzig S, Lee SS, Banting G.
Monitoring of protein secretion with green fluorescent protein. (11-9)
Kaether C, Gerdes HH.
Green fluorescent protein to visualize cancer progression and metastasis. (20-31)
Hoffman RM.
Comparison of enhanced green fluorescent protein and its destabilized form as transcription reporters. (32-8)
Zhao X, Duong T, Huang CC, Kain SR, Li X.
Early detection of apoptosis with annexin V-enhanced green fluorescent protein. (38-43)
Kain SR, Ma JT.
Green fluorescent protein in the visualization of particle uptake and fluid-phase endocytosis. (43-50)
Maniak M.
Monitoring intracellular shutting of histidine-rich pH sensor proteins tagged with green fluorescent protein. (51-8)
Hanakam F, Gerisch G.
Measuring protein degradation with green fluorescent protein. (58-73)
Cronin SR, Hampton RY.
Studying nuclear receptors with green fluorescent protein fusions. (73-84)
Hager GL.
Localization of calmodulin in budding yeast and fission yeast using green fluorescent protein. (87-102)
Flory MR, Davis TN.
Analysis of microtubule organization and dynamics in living cells using green fluorescent protein-microtubule-associated protein 4 chimeras. (103-20)
Olson KR, Olmsted JB.
Trafficking of the androgen receptor. (121-35)
Georget V, Terouanne B, Nicolas JC, Sultan C.
Use of green fluorescent protein for visualization for cell-specific gene expression and subcellular protein localization in Bacillus subtilis. (136-53)
Webb CD, Resnekov O.
Signaling, desensitization, and trafficking of G protein-coupled receptors revealed by green fluorescent protein conjugates. (153-71)
Barak LS, Zhang J, Ferguson SS, Laporte SA, Caron MG.
Use of green fluorescent proteins linked to cytoskeletal proteins to analyze myofibrillogenesis in living cells. (171-86)
Dabiri GA, Ayoob JC, Turnacioglu KK, Sanger JM, Sanger JW.
Fluorescent proteins in single- and multicolor flow cytometry. (189-99)
Lybarger L, Chervenak R.
Flow cytometric analysis of transcription: use of green fluorescent protein variants to control transfection efficiency. (199-206)
Lybarger L, Chervenak R.
Use of coexpressed enhanced green fluorescent protein as a marker for identifying transfected cells. (207-12)
Fang Y, Huang CC, Kain SR, Li X.
Jellyfish green fluorescent protein: a tool for studying ion channels and second-messenger signaling in neurons. (213-25)
Blair LA, Bence KK, Marshall J.
Expression of green fluorescent protein and inositol 1,4,5-triphosphate receptor in Xenopus laevis oocytes. (225-33)
Miyawaki A, Matheson JM, Sayers LG, Muto A, Michikawa T, Furuichi T, Mikoshiba K.
Expression of green fluorescent protein in transgenic mice. (233-50)
Takada T, Yoshida K, Nakamura K, Nakao K, Tsujimoto G, Katsuki M, Sugano S.
Green fluorescent protein as a probe to study intracellular solute diffusion. (250-64)
Verkman AS.
Application of green fluorescent protein-protein A fusion protein to western blotting. (264-72)
Aoki T, Koch KS, Leffert HL, Watabe H.
Green fluorescent protein as a reporter for promoter analysis of testis-specific genes in transgenic mice. (272-84)
Reddi PP, Kallio M, Herr JC.
Green fluorescent protein in the measurement of bacteria-host interactions. (285-95)
Bermudez LE, Sangari FJ, Parker A.
Flow cytometric analysis of transgene expression in higher plants: green fluorescent protein. (296-315)
Galbraith DW, Herzenberg LA, Anderson MT.
Continual green fluorescent protein monitoring of promoter activity in plants. (316-28)
Urwin PE, Moller SG, Blumsom JK, Atkinson HJ.
Retroviral expression of green fluorescent protein. (329-41)
Mazo IA, Levy JP, Muldoon RR, Link CJ Jr, Kain SR.
Confocal imaging of Ca2+, pH, electrical potential, and membrane permeability in single living cells. (341-58)
Lemasters JJ, Trollinger DR, Qian T, Cascio WE, Ohata H.
In vivo retroviral transduction and expression of green fluorescent protein. (358-69)
Levy JP, Muldoon RR, Mazo IA, Kain SR, Link CJ Jr.
S147P green fluorescent protein: a less thermosensitive green fluorescent protein variant. (373-8)
Kimata Y, Lim CR, Kohno K.
Spectral variants of green fluorescent protein. (378-94)
Palm GJ, Wlodawer A.
Expression of green fluorescent protein using baculovirus vectors. (394-408)
King LA, Thomas CJ, Wilkinson N, Possee RD.
Green fluorescent protein forms for energy transfer. (408-23)
Heim R.
Use of codon-modified, red-shifted variants of green fluorescent protein genes to study virus-mediated gene transfer. (424-37)
Link CJ Jr, Wang S, Muldoon RR, Seregina T, Levy JP.
Generation of a destablilized form of enhanced green fluorescent protein. (438-44)
Zhao X, Jiang X, Huang CC, Kain SR, Li X.
Special properties of green fluorescent protein-S65A. (444-9) Inouye S, Umesono K, Tsuji FI.

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